LI Kunyi, LI Sen. Effect of crowded environment on unfolding of alkaline phosphatase[J]. Journal of Beijing Normal University(Natural Science), 2021, 57(4): 485-489. DOI: 10.12202/j.0476-0301.2020408
Citation: LI Kunyi, LI Sen. Effect of crowded environment on unfolding of alkaline phosphatase[J]. Journal of Beijing Normal University(Natural Science), 2021, 57(4): 485-489. DOI: 10.12202/j.0476-0301.2020408
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Effect of crowded environment on unfolding of alkaline phosphatase

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  • The effect of macromolecular crowding agent dextran 70 and osmolyte sucrose on unfolding of alkaline phosphatase was studied by monitoring changes in enzymatic activity and fluorescence emission spectra．Both dextran 70 and sucrose were found to stabilize the activity and conformation of alkaline phosphatase in GdmCl-induced unfolding．Protection against GdmCl-induced unfolding afforded by sucrose was stronger than by dextran 70．
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